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Cloning of a novel xylanase gene from a newly isolated Fusarium sp. Q7-31 and its expression in Escherichia coli BJM
Xie,Zhan-Ling; Gao,Hai-Yan; Zhang,Qian; Wang,Huan; Liu,Ying.
A strain of Q7-31 was isolated from Qinghai-Tibet Plateau and was identified as Fusarium sp. based on its morphological characteristics and ITS rDNA gene sequence analysis. It has the highest capacity of degrading cell wall activity compared with other 11 strains. To do research on its xylanase activity of Fusarium sp. Q7-31 while the degrading the rice cell walls, the complete gene xyn8 that encodes endo-1, 4-β-xylanase secreted by Fusarium sp. Q7-31 was cloned and sequenced. The coding region of the gene is separated by two introns of 56bp and 55bp. It encodes 230 amino acid residues of a protein with a calculated molecular weight of 25.7 kDa. The animo acids sequence of xyn8 gene has higher similarity with those of family 11 of glycosyl hydrolases...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Xylanase; Gene cloning; Fusariumsp.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100049
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Comparative genetic diversity and genetic structure of three chinese silkworm species Bombyx mori L. (Lepidoptera: Bombycidae), Antheraea pernyi Guérin-Meneville and Samia cynthia ricini donovan (Lepidoptera: Saturniidae) Neotropical Entomology
Liu,Yan-Qun; Qin,Li; li,Yu-Ping; Wang,Huan; Xia,Run-Xi; Qi,Yong-Hong; li,Xi-Sheng; Lu,Cheng; Xiang,Zhong-Huai.
The genetic diversity and genetic structure of three Chinese silkworm species Bombyx mori L., Antheraea pernyi Guérin-Meneville and Samia cynthia ricini Donovan were comparatively assessed based on RAPD markers. At the species level, A. pernyi and B. mori showed high levels of genetic diversity, whereas S. cynthia ricini showed low level of genetic diversity. However, at the strain level, A. pernyi had relatively highest genetic diversity and B. mori had lowest genetic diversity. Analysis of molecular variance (AMOVA) suggested that 60% and 72% of genetic variation resided within strains in A. pernyi and S. cynthia ricini, respectively, whereas only 16% of genetic variation occurred within strains in B. mori. In UPGMA dendrogram, individuals of A. pernyi...
Tipo: Info:eu-repo/semantics/article Palavras-chave: RAPD; Genetic variation; AMOVA; Dendrogram.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000600019
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Postnatal Variation of Limb Bones in the Japanese Quail, Coturnix coturnix japonica International Journal of Morphology
Ren,Yaru; Wang,Huan; Zhang,Zihui.
Changes in body size over ontogeny may influence the ontogenetic development of long bones, and thus important to our understanding of variation in morphological, physiological, and life-history traits within species. In this study, we sample the entire measurements of the Japanese quail (Coturnix coturnix japonica) of individual skeletons, to investigate the ontogenetic allometry of limb bone proportions by Reduced Major Axis (RMA) regression. The ulna and humerus were both positively allometric in relation to body mass, with their proximal ends growth more rapidly than other regions. Hindlimb bones exhibited more than one allometric pattern. The tarsometatarsus was negative; the femur presented positive allometry, with the width and depth of the proximal...
Tipo: Journal article Palavras-chave: Ontogenetic allometry; Forelimb; Hindlimb; Japanese quail; Precocial.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200045
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